Tients with diabetes. Solutions: Patients at Concord CD59 Proteins medchemexpress Hospital with suspected CAD gave written informed consent and were administered RIPC (sphygmomanometer on the arm, 3 five min cycles, n = 31) or sham (n = 29) prior to angiography, with recruitment ongoing. Blood was collected pre- and straight away post-RIPC/sham and plateletfree plasma generated. Global coagulation/fibrinolytic potential was measured by all round haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and several fibrinolytic aspects by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Study ICAM-1/CD54 Proteins site Institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have prospective as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying bring about of heart attack and stroke, EV release may be dysregulated and their contents can mediate pro-inflammatory effects. Many markers have been previously identified on uEV including exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of those membrane bound carriers involve microRNAs (miRs). miR-21 and miR-155 are essential regulatory miRs which can be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to have pro-atherogenic effects and miR-155 deficiency in murine models results in reduced atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from patients diagnosed with coronary artery illness (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic individuals had been isolated by means of benchtop centrifugation. The concentration and size of uEVs have been analysed via the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = ten per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Results: uEV concentration in symptomatic sufferers (median; six.46E+9 particles/mL) was significantly decreased (p 0.05) in comparison to asymptomatic individuals (median; 1.25E+10 particles/mL). CD11B+ uEVs were enhanced and CD16+ uEVs have been decreased within the symptomatic individuals (p 0.01). Also, the concentration of CD45+ EVs have been improved in symptomatic sufferers (p 0.001). Although uEV miR-21 was unchanged, miR-155 expression was significantly increased within the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic prospective. As CAD severity increases, uEV concentration is lowered, surface marker expression is altered and uEV miR-155 expression is enhanced. Funding: The Irish Investigation Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Wellness Evaluative Sciences, Research Institute, The Hospital for Sick Kids,.