T developments in ionization strategies such as MALDI-2 and their application as an illustration in redox lipidomics, deliver an exciting chance to study rare lipids [726, 727]. In view with the ongoing shift in IL-1 Proteins Molecular Weight Cancer research towards IL-13 Receptor Proteins Source single cell approaches revealing the value of tissue heterogeneity in cancer progression and therapeutic outcome, we are going to witness a shift in the lipidomics field from lipidomics analyses on bulk tissue to single cell and spatial analysis by mass spectrometry imaging. New technological developments in this domain guarantee an unseen efficiency when it comes to analytical elements too as spatial resolution, top to novel insights in the function of lipids in the complicated metabolic interplay in between diverse cell sorts inside the heterogenous tumor microenvironment. With novel technologies permitting imaging of lipids in the intracellular level which includes dynamic SIMS ion microcopy and Raman microscopy, a whole new location of lipid investigation is opening up, revealing adjustments in organellar lipidomes, trafficking pathways and membrane structures [728, 729]. The additional improvement of steady isotope lipidomics will let to follow adjustments in pathway fluxes as an alternative of present steady state analysis and collectively with spatial multi-omics approaches will provide unprecedented insight in affected pathways and possible biomarkers. Together with these developments there’s an urgent want for standardization of strategies and technologies to let future clinical implementations with the found biomarkers. When it comes to therapeutic prospective, existing findings suggest that interference with lipid metabolism may have promising applications, particularly in combinatorial approaches[16]. With little molecules targeting enzymes in lipid metabolism entering clinical trials we are at the doorstep of witnessing the clinical exploitation of altered lipid metabolism as a hallmark of cancer. The hyperlink with the diet plan, such as dietary lipids may also develop unique opportunities for preventive approaches and therapy enhancement. Particularly within the field of tumor immunology, lipids hold excellent potential as modulators. In summary, despite the fact that lagging behind when compared with other omics approaches, the study of lipids in cancer is swiftly catching up and is establishing itself as a central hallmark of cancer with promising possibilities for clinical application.Author Manuscript Author Manuscript Author Manuscript Funding Author ManuscriptThis work was supported by an EU Interreg grant V-A EMR23 EURLIPIDS, KU Leuven grants C1 (C16/15/073) and C3 (C32/17/052), Analysis Foundation-Flanders (FWO), Stichting tegen Kanker, Kom op tegen Kanker, the Movember Foundation/Prostate Cancer Foundation of Australia (MRTA3), The Prostate Cancer Foundation of Australia (ID NDDA), the Cancer Council South Australia Beat Cancer Project, NIH grant RO1CA58961, a Norris Cotton Cancer Center grant, as well as the Dartmouth College Norris Cotton Cancer Center Help Grant P30CA023108. ML’s function is supported by NIH grants RO1CA131945, R01CA187918, DoD PC160357, DoD PC180582, P50CA211024, as well as the Prostate Cancer Foundation.AbbreviationsAA ACACA arachidonic acid acetyl-CoA carboxylase alphaAdv Drug Deliv Rev. Author manuscript; offered in PMC 2021 July 23.Butler et al.PageACACBacetyl-CoA carboxylase beta ATP-citrate lyase Acyl-CoA Synthetase acylprotein thioesterase androgen receptor acid sphingomyelinase adipose triglyceride lipase autotaxin breast cancer clear cell renal cell carcinoma chol.