We hypothesized that the certain proteins containing EV released from stroke serum cultured MSCs may possibly influence the neurogenesis and angiogenesis of recipient cells. Methods: EVs were purified from conditioned media of MSC cultured with FBS (FBS-MSC) and MSC cultured with stroke serum (SS-MSC). These EVs were characterized by nanoparticle tracking evaluation. EV protein profiling in conditioned media was systematically compared by way of using LC-MS/MS-based label-free quantification. Real-time PCR was performed to decide the difference in the gene Nav1.2 Purity & Documentation expression in each cell. The protein concentration within the EV was confirmed by ELISA. Final results: A total of 1068 proteins had been identified from SS-MSC-EV and FBS-MSC-EV through LC-MS. According to statistical analysis, 22 proteins were found to become extra than 2-fold (p 0.05) upregulated in SS-MSC_EV. ITGA5, CLU, and CTSB were considerably enhanced of SS-MSC gene expression levels when compared with FBS-MSC. Among the candidate proteins, clusterin (CLU) was found to be upregulated in EVs from SS-MSC when compared with those from FBS-MSC. Summary/Conclusion: These benefits recommend that SSMSC_EVs containing clusterin may possibly promote intercellular communication and influence neurogenesis and angiogenesis of recipient cells. Funding: This study was supported by a grant from the Korean Healthcare Technology R D Project, Ministry of Overall health Welfare (HI17C1256) and Fundamental Science Study Program, the Ministry of Science, ICT and Future Planning (2018M3A9H1023675).PT10.Adipose-derived Stem/Stromal Cell secretome, containing each soluble things and extracellular vesicles, exerts chondroprotective effects in vitro Chiara Giannasia, Stefania Niadaa, Sara Casatib and Anna BrinicaPT10.Proteomic analysis of extracellular vesicles from MSC cultured with stroke serum Yeon Hee Choa, Eun Hee Kima, Dong Hee Kimb, Ji Hee Sunga, Mi Jeong Oha, Eun Kyoung Shina and Oh Young BangaaIRCCS Istituto Ortopedico Galeazzi, Milano, Italy; bDepartment of Biomedical, Surgical and Dental Sciences, University of Milan, Milano, Italy; cDepartment of Biomedical, Surgical and Dental Sciences, University of Milan. IRCCS Istituto Ortopedico Galeazzi, Milano, ItalySamsung Healthcare Center, Seoul, Republic of Korea; University, Seoul, Republic of KoreabSungkyunkwanIntroduction: Serum from stroke sufferers increases mesenchymal stem cells trophism towards the infarctedIntroduction: Up to now various clinical trials have shown the safety and efficacy with the intra-articular injection of Adipose-derived Mesenchymal Stem/ Stromal Cells (ASCs) in contrasting osteoarthritis.MMP-3 Formulation ISEV2019 ABSTRACT BOOKSince ASCs act predominantly through paracrine mechanisms, their secretome represents a promising cell-free alternative. Here we identified anti-hypertrophic and anti-catabolic effects of ASC conditioned medium (ASC-CM) on TNF-stimulated human primary articular chondrocytes (CHs). Solutions: CHs were treated with 10 ng/mL TNF and/ or ASC-CM administered at a 1:five recipient:donor cell ratio. Cell viability was assessed up to day 9. The activity, expression and/or release of hypertrophy markers (MMP-13, Collagen X and Osteocalcin), catabolic mediators (MMP-3) and cartilage-protective variables have been assessed as much as day three by enzymatic assays, qRTPCR, Western Blot and multiplex immunoassays. Results: ASC-CM blunted TNF-induced hypertrophy, reducing the enhanced levels of MMP-13 activity (-61), Osteocalcin (-37) and Collagen X (-18). Moreover, also MMP-3 activity was diminished by -59.