Of conjugates that may be readily prepared. Now, researchers can quickly screen a large number of interesting conjugates that were chemically difficult, if not impossible, before, and do it in a controlled, single step fashion that enhances the probability of success. The fact that the conjugation chemistry is accomplished while the oligonucleotide is still support-bound adds several advantages. Often, successful conjugations are limited to those that are amenable to the requirement that some water (20-50%) is needed to dissolve the oligonucleotide in the conjugation mixture. Solid phase conjugations allow the use of completely organic systems, even dichloromethane, for conjugation. This allows the ready conjugation of very lipophilic compounds to oligonucleotides, which can then act as purification handles for reverse phase purification of the conjugates. Another advantage of this system is that the reagent used in excess, the amine, is not affected by the reaction and therefore recoverable. In fact, the reagent can be used again without further purification for the next conjugation reaction. Besides the obvious cost savings, this will also improve overall efficiency in that much larger excesses are now feasible even in large-scale conjugations. By driving the reaction further to completion, downstream operations such as purifications are reduced or even eliminated. 5′-Carboxy-Modifier C10 is offered for sale under license from TriLink BioTechnologies, Inc. It is intended for research and development purposes only, and may not be used for commercial, clinical, diagnostic or any other use. It is covered under US Patent No. 6,320,041.
Wash the support with acetonitrile and air dry it. Add the support to a vial containing 10 equivalents of the amine dissolved in 2 mL of dichloromethane or other appropriate solvent with 10% triethylamine. Leave the reaction at room temperature for 4 hours with continuous agitation. Decant the amine solution from the support and wash with further dichloromethane.67469-78-7 site Air dry the support.885060-09-3 web Proceed to regular deprotection, keeping in mind the stability of the modified oligonucleotide to the deprotection conditions.PMID:30521237
fluorescence of a nucleoside base is highly dependent on the environment of the base and the measurement of its fluorescence is a powerful and sensitive tool for the analysis of DNA and RNA structure. It is especially useful for analyzing the interaction of DNA and RNA with their corresponding binding proteins. Fluorescence measurements allow real-time probing of these structural interactions. Unfortunately, the intrinsic fluorescence of the regular bases is extremely low to non-existent, so fluorescent analogues have been sought for a long time. For probing DNA structure, the ideal fluorescent nucleoside: Should have bright fluorescence, which is sensitive to its environment, and a large Stokes shift; Should be amenable to phosphoramidite preparation for incorporation into oligonucleotides by solid-phase synthesis; Should not disrupt duplex formation and should mimic one of the regular nucleosides; Should behave as a regular nucleoside in its interaction with proteins and enzymes; and Should be capable of being converted to the triphosphate and be incorporated into DNA with high efficiency by current commercial polymerases.
It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same Tm as the control dC oligo with the same specificity for dG. Its small size does not p.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com